References for anti-Asialoglycoprotein Receptor 1 antibody (ABIN2688844) (hide) Touboul, Hannan, Corbineau, Martinez, Martinet, Branchereau, Mainot, Strick-Marchand, Pedersen, Di Santo, Weber, Vallier: " Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development." in: Hepatology (Baltimore, Md.), Vol.This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.Īqueous buffered solution containing ≤0.09 % sodium azide. The second-step reagent was Alexa Fluor® 488 Goat Anti-Mouse IgG (Life Technologies), and counter-staining was with DAPI (4',6-Diamidino-2-phenylindole dihydrochloride, pseudo-colored blue). Hep G2 cells (ATCC, HB-8065) were fixed with BD Cytofix™ Fixation Buffer and stained with Purified Mouse Anti-ASGPR 1 (pseudo-colored green) at 5 μg/mL. Immunofluorescent Staining of ASGPR 1 in Human Hepatocellular Carcinoma (Hep G2) cells. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.įlow Cytometry System. The 8D7 antibody has been shown to react with a subunit-specific epitope on RHL-1.īD Pharmingen™ Purified Mouse Anti-ASGPR 1 - Purified - Clone 8D7 - Isotype Mouse IgG1, κ - Reactivity Hu, Rat - 0.1 mg Rat ASGPR consists of three polypeptide subunits (Rat hepatic lectin 1-3 (RHL1-3). The immunogen used to generate the 8D7 hybridoma was rat liver membrane extracts. The receptor may also promote hepatic infection by the binding and uptake of various viruses. It functions as a subunit of the Asialoglycoprotein receptor (ASGPR) complex that binds, internalizes, and transports various glycoproteins for lysosomal degradation. ASGPR 1 plays a role in serum glycoprotein homeostasis. It is expressed by hepatocytes on the sinusoidal-lateral plasma membrane but not on the bile canalicular membrane. ASGPR 1 is an approximately 42 kDA type II integral membrane protein that is expressed on the surface of hepatic cells. The 8D7 monoclonal antibody recognizes Asialoglycoprotein receptor 1 (ASGPR 1), also known as Hepatic lectin H1 (HL-1). Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), BioImaging (BI) Immunofluorescence (fixed cells) (IF/ICC) Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) Immunohistochemistry (Frozen Sections) (IHC (fro)) Immunofluorescence (Paraffin-embedded Sections) (IF (p))
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